Search results for "FLAVESCENCE DOREE"
showing 8 items of 8 documents
Multilocus sequence typing confirms the close genetic inter-relatedness between three distinct flavescence doree phytoplasma strain clusters and grou…
2007
ABSTRACT Vineyards of southern France and northern Italy are affected by the flavescence dorée (FD) phytoplasma, a quarantine pathogen transmitted by the leafhopper of Nearctic origin Scaphoideus titanus . To better trace propagation of FD strains and identify possible passage between the vineyard and wild plant compartments, molecular typing of phytoplasma strains was applied. The sequences of the two genetic loci map and uvrB - degV , along with the sequence of the secY gene, were determined among a collection of FD and FD-related phytoplasmas infecting grapevine, alder, elm, blackberry, and Spanish broom in Europe. Sequence comparisons and phylogenetic analyses consistently indicated the…
A chromosome map of the Flavescence dorée phytoplasma
2008
International audience; The Flavescence dorée phytoplasma (FD-P), a non-cultivable, plant-pathogenic bacterium of the class Mollicutes, is the causal agent of a quarantine disease affecting vineyards of southern Europe, mainly in southern France and northern Italy. To investigate FD-P diversity and phytoplasma genetic determinants governing the FD-P life cycle, a genome project has been initiated. A physical map of the chromosome of FD-P strain FD92, purified from infected broad beans, was constructed by performing restriction digests of the chromosome and resolving the fragments by PFGE. Single and double digestions of the chromosome with the enzymes SalI, BssHII, MluI and EagI were perfor…
Génomique du phytoplasme de la Flavescence Dorée
2008
National audience
Epidémiologie moléculaire et diversité des phytoplasmes du groupe 16SrV présents dans les compartiments viticoles et sauvages en Europe
2008
National audience
Progress towards a chromosome map of the “flavescence dorée” phytoplasma
2007
International audience
Map gene as a new non ribosomal marker to study the molecular variability of grapevine yellows and related phytoplasmas from group 16SRV in Europe
2006
International audience
Sequence typing of map, secY and degV genes confirm the genetic relatedness of three distinct groups of flavescence dorée phytoplasmas with phytoplas…
2006
International audience
Improved detection of flavescence dorée and related phytoplasma in the elm yellows group in difficult material, with specific PCR primers that amplif…
2000
Molecular-based methods are sometimes not sensitive enough to detect low-titre phytoplasmas in woody hosts. In order to enable the reliable routine diagnosis of Grapevine Yellows in dormant grapevine material, INRA-Dijon constructed new FD9 primers which, used in one step-PCR, proved very effective for the detection of phytoplasmas in symptomatic grapevine and elm. Used in nested-PCR assays, they provided an excellent signal from phloem chips or whole tissues taken from either canes or roots of dormant plants. Their restriction patterns were shown to be as informative as those from the original FD9 product. The new primers will help in the development of sanitary certification protocols and…